HIBADH 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
HIBADH transfected lysate.
Western Blot
Lane 1: HIBADH transfected lysate ( 37.07 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-HIBADH full-length
Host
Human
Theoretical MW (kDa)
37.07
Interspecies Antigen Sequence
Mouse (91); Rat (91)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-HIBADH antibody (H00011112-B01) by Western Blots.
SDS-PAGE Gel
HIBADH transfected lysate.
Western Blot
Lane 1: HIBADH transfected lysate ( 37.07 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — HIBADH
Entrez GeneID
11112GeneBank Accession#
NM_152740.2Protein Accession#
NP_689953.1Gene Name
HIBADH
Gene Alias
MGC40361, NS5ATP1
Gene Description
3-hydroxyisobutyrate dehydrogenase
Omim ID
608475Gene Ontology
HyperlinkGene Summary
3-hydroxyisobutyrate dehydrogenase (3-hydroxy-2-methylpropanoate:NAD(+) oxidoreductase, EC 1.1.1.31) is a dimeric mitochondrial enzyme that catalyzes the NAD(+)-dependent, reversible oxidation of 3-hydroxyisobutyrate, an intermediate of valine catabolism, to methylmalonate semialdehyde.[supplied by OMIM
Other Designations
3'-hydroxyisobutyrate dehydrogenase
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Interactome
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Pathway
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