KERA (Human) Recombinant Protein (Q01)
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More Files
- More Functions
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Specification
Product Description
Human KERA partial ORF ( NP_008966, 253 a.a. - 351 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
GLPSRGFDVSSILDLQLSHNQLTKVPRISAHLQHLHLDHNKIKSVNVSVICPSPSMLPAERDSFSYGPHLRYLRLDGNEIKPPIPMALMTCFRLLQAVI
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
36.63
Interspecies Antigen Sequence
Mouse (85); Rat (86)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — KERA
Entrez GeneID
11081GeneBank Accession#
NM_007035Protein Accession#
NP_008966Gene Name
KERA
Gene Alias
CNA2, SLRR2B
Gene Description
keratocan
Gene Ontology
HyperlinkGene Summary
Keratan sulfate proteoglycans (KSPGs) are members of the small leucine-rich proteoglycan (SLRP) family. KSPGs, particularly keratocan, lumican (MIM 600616), and mimecan (MIM 602383), are important to the transparency of the cornea.[supplied by OMIM
Other Designations
cornea plana 2 (autosomal recessive)|keratocan proteoglycan
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Interactome
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Publication Reference
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Resistance of Corneal RFUVA-Cross-Linked Collagens and Small Leucine-Rich Proteoglycans to Degradation by Matrix Metalloproteinases.
Zhang Y, Mao X, Schwend T, Littlechild S, Conrad GW.
Investigative Ophthalmology & Visual Science 2013 Feb; 54(2):1014.
Application:Substrate, Bovin, Enzyme.
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Effects of Ultraviolet-A and Riboflavin on the Interaction of Collagen and Proteoglycans during Corneal Cross-linking.
Zhang Y, Conrad AH, Conrad GW.
The Journal of Biological Chemistry 2011 Apr; 286(15):13011.
Application:PI, WB-Re, Recombinant protein.
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Resistance of Corneal RFUVA-Cross-Linked Collagens and Small Leucine-Rich Proteoglycans to Degradation by Matrix Metalloproteinases.
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