DNAJB4 monoclonal antibody (M02), clone S13
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant DNAJB4.
Immunogen
DNAJB4 (AAH34721, 1 a.a. ~ 337 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MGKDYYCILGIEKGASDEDIKKAYRKQALKFHPDKNKSPQAEEKFKEVAEAYEVLSDPKKREIYDQFGEEGLKGGAGGTDGQGGTFRYTFHGDPHATFAAFFGGSNPFEIFFGRRMGGGRDSEEMEIDGDPFSAFGFSMNGYPRDRNSVGPSRLKQDPPVIHELRVSLEEIYSGCTKRMKISRKRLNADGRSYRSEDKILTIEIKKGWKEGTKITFPREGDETPNSIPADIVFIIKDKDHPKFKRDGSNIIYTAKISLREALCGCSINVPTLDGRNIPMSVNDIVKPGMRRRIIGYGLPFPKNPDQRGDLLIEFEVSFPDTISSSSKEVLRKHLPAS
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (94); Rat (95)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (62.81 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of DNAJB4 expression in transfected 293T cell line by DNAJB4 monoclonal antibody (M02), clone S13.
Lane 1: DNAJB4 transfected lysate(37.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of DNAJB4 transfected lysate using anti-DNAJB4 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with DNAJB4 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged DNAJB4 is 0.3 ng/ml as a capture antibody.ELISA
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Gene Info — DNAJB4
Entrez GeneID
11080GeneBank Accession#
BC034721Protein Accession#
AAH34721Gene Name
DNAJB4
Gene Alias
DNAJW, DjB4, HLJ1
Gene Description
DnaJ (Hsp40) homolog, subfamily B, member 4
Omim ID
611327Gene Ontology
HyperlinkGene Summary
DNAJB4 belongs to the evolutionarily conserved DNAJ/HSP40 protein family. For background information on the DNAJ family, see MIM 608375.[supplied by OMIM
Other Designations
DnaJ-like heat shock protein 40|OTTHUMP00000038541
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