RAPGEF4 monoclonal antibody (M01), clone 1C11
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More Files
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Specifications
Product Description
Mouse monoclonal antibody raised against a partial recombinant RAPGEF4.
Immunogen
RAPGEF4 (AAH24004, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MVAAHAAHSSSSAEWIACLDKRPLERSSEDVDIIFTRLKEVKAFEKFHPNLLHQICLCGYYENLEKGITLFRQGDIGTNWYAVLAGSLDVKVSETSSHQDAVTICTLGIG
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (97)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.73 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of RAPGEF4 expression in transfected 293T cell line by RAPGEF4 monoclonal antibody (M01), clone 1C11.
Lane 1: RAPGEF4 transfected lysate (Predicted MW: 115.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RAPGEF4 is approximately 0.3ng/ml as a capture antibody.ELISA
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Gene Info — RAPGEF4
Entrez GeneID
11069GeneBank Accession#
BC024004Protein Accession#
AAH24004Gene Name
RAPGEF4
Gene Alias
CAMP-GEFII, CGEF2, EPAC2, Nbla00496
Gene Description
Rap guanine nucleotide exchange factor (GEF) 4
Omim ID
606058Gene Ontology
HyperlinkOther Designations
OTTHUMP00000165043|OTTHUMP00000165045|RAP guanine-nucleotide-exchange factor (GEF) 4|cAMP-regulated guanine nucleotide exchange factor II|exchange protein directly activated by cAMP 2|putative protein product of Nbla00496
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Interactomes
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Pathways
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Diseases
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