UBE2C monoclonal antibody (M04), clone 3B1
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of UBE2C expression in transfected 293T cell line by UBE2C monoclonal antibody (M04), clone 3B1.
Lane 1: UBE2C transfected lysate(19.7 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of monoclonal antibody to UBE2C on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (45.43 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant UBE2C.
Immunogen
UBE2C (AAH50736, 1 a.a. ~ 179 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MASQNRDPAATSVAAARKGAEPSGGAARGPVGKRLQQELMTLMMSGDKGISAFPESDNLFKWVGTIHGAAGTVYEDLRYKLSLEFPSGYPYNAPTVKFLTPCYHPNVDTQGNICLDILKEKWSALYDVRTILLSIQSLLGEPNIDSPLNTHAAELWKNPTAFKKYLQETYSKQVTSQEP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (95); Rat (96)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (45.43 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of UBE2C expression in transfected 293T cell line by UBE2C monoclonal antibody (M04), clone 3B1.
Lane 1: UBE2C transfected lysate(19.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to UBE2C on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — UBE2C
Entrez GeneID
11065GeneBank Accession#
BC050736Protein Accession#
AAH50736Gene Name
UBE2C
Gene Alias
UBCH10, dJ447F3.2
Gene Description
ubiquitin-conjugating enzyme E2C
Omim ID
605574Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000031653|OTTHUMP00000031655|cyclin-selective ubiquitin carrier protein|mitotic-specific ubiquitin-conjugating enzyme|ubiquitin carrier protein E2-C|ubiquitin-protein ligase C
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Interactome
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Pathway
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Disease
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