WWP1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
WWP1 transfected lysate.
Western Blot
Lane 1: WWP1 transfected lysate ( 105.20 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-WWP1 full-length
Host
Human
Theoretical MW (kDa)
105.2
Interspecies Antigen Sequence
Mouse (90); Rat (90)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-WWP1 antibody (H00011059-D01P) by Western Blots.
SDS-PAGE Gel
WWP1 transfected lysate.
Western Blot
Lane 1: WWP1 transfected lysate ( 105.20 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — WWP1
Entrez GeneID
11059GeneBank Accession#
NM_007013.3Protein Accession#
NP_008944.1Gene Name
WWP1
Gene Alias
AIP5, DKFZp434D2111, Tiul1, hSDRP1
Gene Description
WW domain containing E3 ubiquitin protein ligase 1
Omim ID
602307Gene Ontology
HyperlinkGene Summary
WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. The encoded protein belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. Alternative splicing of this gene generates at least 6 transcript variants; however, the full length nature of these transcripts has not been defined. [provided by RefSeq
Other Designations
Nedd-4-like ubiquitin-protein ligase|TGIF-interacting ubiquitin ligase 1|atrophin-1 interacting protein 5
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Interactome
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