ADRM1 monoclonal antibody (M01), clone 3C6
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More Files
- More Functions
Western Blot (Cell lysate)
ADRM1 monoclonal antibody (M01), clone 3C6 Western Blot analysis of ADRM1 expression in HeLa ( Cat # L013V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of ADRM1 expression in transfected 293T cell line by ADRM1 monoclonal antibody (M01), clone 3C6.
Lane 1: ADRM1 transfected lysate(42.2 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of ADRM1 transfected lysate using anti-ADRM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ADRM1 monoclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ADRM1 is approximately 0.1ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to ADRM1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (68.64 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant ADRM1.
Immunogen
ADRM1 (AAH17245.1, 18 a.a. ~ 407 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
ASNKYLVEFRAGKMSLKGTTVTPDKRKGLVYIQQTDDSLIHFCWKDRTSGNVEDDLIIFPDDCEFKRVPQCPSGRVYVLKFKAGSKRLFFWMQEPKTDQDEEHCRKVNEYLNNPPMPGALGASGSSGHELSALGGEGGLQSLLGNMSHSQLMQLIGPAGLGGLGGLGALTGPGLASLLGSSGPPGSSSSSSSRSQSAAVTPSSTTSSTRATPAPSAPAAASATSPSPAPSSGNGASTAASPTQPIQLSDLQSILATMNVPAGPAGGQQVDLASVLAPEIMAPILANADVQERLLPYLPSGESLPQTADEIQNTLTSPQFQQALGMFSAALASGQLGPLMCQFGLPAEAVEAANKGDVEAFAKAMQNNAKPEQKEGDTKDKKDEEEDMSLD
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (96); Rat (96)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (68.64 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
ADRM1 monoclonal antibody (M01), clone 3C6 Western Blot analysis of ADRM1 expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of ADRM1 expression in transfected 293T cell line by ADRM1 monoclonal antibody (M01), clone 3C6.
Lane 1: ADRM1 transfected lysate(42.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of ADRM1 transfected lysate using anti-ADRM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ADRM1 monoclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ADRM1 is approximately 0.1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to ADRM1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — ADRM1
Entrez GeneID
11047GeneBank Accession#
BC017245Protein Accession#
AAH17245.1Gene Name
ADRM1
Gene Alias
ARM1, GP110, MGC29536, Rpn13
Gene Description
adhesion regulating molecule 1
Omim ID
610650Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an integral plasma membrane protein which promotes cell adhesion. The encoded protein is thought to undergo O-linked glycosylation. Expression of this gene has been shown to be induced by gamma interferon in some cancer cells. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
110 kDa cell membrane glycoprotein|M(r) 110,000 surface antigen|OTTHUMP00000031471|regulatory particle non-ATPase 13
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Interactome
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Publication Reference
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Phospho-ΔNp63α/Rpn13-dependent regulation of LKB1 degradation modulates autophagy in cancer cells.
Huang Y, Ratovitski EA.
Aging 2010 Dec; 2(12):959.
Application：WB, Human, Squamous carcinoma (SCC) stable cell lines.
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Phosphorylated TP63 Induces Transcription of RPN13, Leading to NOS2 Protein Degradation.
Huang Y, Ratovitski EA.
The Journal of Biological Chemistry 2010 Dec; 285(53):41422.
Application：WB, Human, Squamous carcinoma (SCC) stable cell lines.
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Biological and Molecular Heterogeneity of Breast Cancers Correlates with Their Cancer Stem Cell Content.
Pece S, Tosoni D, Confalonieri S, Mazzarol G, Vecchi M, Ronzoni S, Bernard L, Viale G, Pelicci PG, Di Fiore PP.
Cell 2010 Jan; 140(1):62.
Application：IF, Human, Human breast cancer tissues.
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Phospho-ΔNp63α/Rpn13-dependent regulation of LKB1 degradation modulates autophagy in cancer cells.
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