MID2 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MID2 full-length
Host
Human
Theoretical MW (kDa)
75.46
Interspecies Antigen Sequence
Mouse (99); Rat (94)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MID2 antibody (H00011043-B01) by Western Blots.
Western Blot
Lane 1: MID2 transfected lysate ( 75.35 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
MID2 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MID2
Entrez GeneID
11043GeneBank Accession#
BC017707Protein Accession#
AAH17707Gene Name
MID2
Gene Alias
FXY2, MID1, RNF60, TRIM1
Gene Description
midline 2
Omim ID
300204Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000023817|dJ820B18.2 (midline 1 (Opitz/BBB syndrome))|midin 2|tripartite motif protein 1
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