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ALF 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00011036-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-ALF full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 52.69
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-ALF antibody (H00011036-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00011036-T01
    ALF transfected lysate.
    Western Blot
    QC Testing of H00011036-T01
    Lane 1: ALF transfected lysate ( 52.69 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Protein Accession#:
  • -
  • Gene Name:
  • GTF2A1L
  • Gene Alias:
  • ALF,MGC26254
  • Gene Description:
  • general transcription factor IIA, 1-like
  • Gene Summary:
  • The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involve the effects of TFIIA on the interaction between TATA-binding protein (TBP) and DNA. This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology. Alternative splicing for this locus has been observed and two variants, encoding distinct isoforms, have been identified. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq
  • Other Designations:
  • GTF2A1-like factor,TFIIA alpha/beta like factor,TFIIA large subunit isoform ALF,TFIIA-alpha/beta-like factor
  • Related Disease
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