ALF monoclonal antibody (M05), clone 3E4
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of ALF expression in transfected 293T cell line by ALF monoclonal antibody (M05), clone 3E4.
Lane 1: ALF transfected lysate(52.4 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of monoclonal antibody to GTF2A1L on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (36.52 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant ALF.
Immunogen
ALF (NP_006863, 251 a.a. ~ 348 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
PQVSQTNSNVESVLSGSASMAQNLHDESLSTSPHGALHQHVTDIQLHILKNRMYGCDSVKQPRNIEEPSNIPVSEKDSNSQVDLSIRVTDDDIGEIIQ
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (71); Rat (73)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.52 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of ALF expression in transfected 293T cell line by ALF monoclonal antibody (M05), clone 3E4.
Lane 1: ALF transfected lysate(52.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to GTF2A1L on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — GTF2A1L
Entrez GeneID
11036GeneBank Accession#
NM_006872Protein Accession#
NP_006863Gene Name
GTF2A1L
Gene Alias
ALF, MGC26254
Gene Description
general transcription factor IIA, 1-like
Omim ID
605358Gene Ontology
HyperlinkGene Summary
The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involve the effects of TFIIA on the interaction between TATA-binding protein (TBP) and DNA. This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology. Alternative splicing for this locus has been observed and two variants, encoding distinct isoforms, have been identified. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq
Other Designations
GTF2A1-like factor|TFIIA alpha/beta like factor|TFIIA large subunit isoform ALF|TFIIA-alpha/beta-like factor
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Interactome
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Pathway
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Disease
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