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KDELR2 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00011014-T02
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-KDELR2 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 24.4
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-KDELR2 antibody (H00011014-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00011014-T02
    KDELR2 transfected lysate.
    Western Blot
    QC Testing of H00011014-T02
    Lane 1: KDELR2 transfected lysate ( 24.40 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • KDELR2
  • Gene Alias:
  • ELP-1,ERD2.2,FLJ45532
  • Gene Description:
  • KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 2
  • Gene Summary:
  • Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. KDELR2 was the second member of the family to be identified, and it encodes a protein which is 83% identical to the KDELR1 gene product. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq
  • Other Designations:
  • (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 2,ERD-2-like protein,KDEL receptor 2
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