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Last updated: 2016/11/27

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SF3B2 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00010992-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-SF3B2 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 70.07
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-SF3B2 antibody (H00010992-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00010992-T01
    SF3B2 transfected lysate.
    Western Blot
    QC Testing of H00010992-T01
    Lane 1: SF3B2 transfected lysate ( 70.07 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • SF3B2
  • Gene Alias:
  • SAP145,SF3B145,SF3b1,SF3b150
  • Gene Description:
  • splicing factor 3b, subunit 2, 145kDa
  • Gene Summary:
  • This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus. [provided by RefSeq
  • Other Designations:
  • pre-mRNA splicing factor SF3b 145 kDa subunit,spliceosome associated protein 145,splicing factor 3B subunit 2
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