SF3B2 purified MaxPab mouse polyclonal antibody (B01P)
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More Files
- More Functions
Western Blot (Cell lysate)
SF3B2 MaxPab polyclonal antibody. Western Blot analysis of SF3B2 expression in Hela S3 NE.
Western Blot (Transfected lysate)
Western Blot analysis of SF3B2 expression in transfected 293T cell line (H00010992-T01) by SF3B2 MaxPab polyclonal antibody.
Lane 1: SF3B2 transfected lysate(69.96 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to SF3B2 on HeLa cell. [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human SF3B2 protein.
Immunogen
SF3B2 (AAH07610.1, 1 a.a. ~ 636 a.a) full-length human protein.
Sequence
MDDPSVGPKIPQALEKILQLKESRQEEMNSQQEEEEMETDARSSLGQSASETEEDTVSVSKKEKNRKRRNRKKKKKPQRVRGVSSESSGDREKDSTRSRGSDSPAADVEIEYVTEEPEIYEPNFIFFKRIFEAFKLTDDVKKEKEKEPEKLDKLENSAAPKKKGFEEEHKDSDDDSSDDEQEKKPEAPKLSKKKLRRMNRFTVAELKQLVARPDVVEMHDVTAQDPKLLVHLKATRNSVPVPRHWCFKRKYLQGKRGIEKPPFELPDFIKRTGIQEMREALQEKEEQKTMKSKMREKVRPKMGKIDIDYQKLHDAFFKWQTKPKLTIHGDLYYEGKEFETRLKEKKPGDLSDELRISLGMPVGPNAHKVPPPWLIAMQRYGPPPSYPNLKIPGLNSPIPESCSFGYHAGGWGKPPVDETGKPLYGDVFGTNAAEFQTKTEEEEIDRTPWGELEPSDEESSEEEEEEESDEDKPDETGFITPADSGLITPGGFSSVPAGMETPELIELRKKKIEEAMDGSETPQLFTVLPEKRTATVGGAMMGSTHIYDMSTVMSRKGPAPELQGVEVALAPEELELDPMAMTQKYEEHVREQQAQVEKEDFSDMVAEHAAKQKQKKRKAQPQDSRGGSKKYKEFKF
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (98); Rat (98)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
SF3B2 MaxPab polyclonal antibody. Western Blot analysis of SF3B2 expression in Hela S3 NE.Western Blot (Transfected lysate)
Western Blot analysis of SF3B2 expression in transfected 293T cell line (H00010992-T01) by SF3B2 MaxPab polyclonal antibody.
Lane 1: SF3B2 transfected lysate(69.96 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to SF3B2 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — SF3B2
Entrez GeneID
10992GeneBank Accession#
BC007610.1Protein Accession#
AAH07610.1Gene Name
SF3B2
Gene Alias
SAP145, SF3B145, SF3b1, SF3b150
Gene Description
splicing factor 3b, subunit 2, 145kDa
Omim ID
605591Gene Ontology
HyperlinkGene Summary
This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus. [provided by RefSeq
Other Designations
pre-mRNA splicing factor SF3b 145 kDa subunit|spliceosome associated protein 145|splicing factor 3B subunit 2
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Interactome
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