RAB32 monoclonal antibody (M01), clone 1C7
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant RAB32.
Immunogen
RAB32 (NP_006825, 136 a.a. ~ 225 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
IPAVLLANKCDQNKDSSQSPSQVDQFCKEHGFAGWFETSAKDNINIEEAARFLVEKILVNHQSFPNEENDVDKIKLDQETLRAENKSQCC
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (84); Rat (83)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.64 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of RAB32 expression in transfected 293T cell line by RAB32 monoclonal antibody (M01), clone 1C7.
Lane 1: RAB32 transfected lysate(25 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of RAB32 transfected lysate using anti-RAB32 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RAB32 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RAB32 is 0.3 ng/ml as a capture antibody.ELISA
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Gene Info — RAB32
Entrez GeneID
10981GeneBank Accession#
NM_006834Protein Accession#
NP_006825Gene Name
RAB32
Gene Alias
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Gene Description
RAB32, member RAS oncogene family
Gene Ontology
HyperlinkGene Summary
Small GTP-binding proteins of the RAB family, such as RAB32, play essential roles in vesicle and granule targeting (Bao et al., 2002 [PubMed 11784320]).[supplied by OMIM
Other Designations
OTTHUMP00000017369
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