PLEKHC1 monoclonal antibody (M09), clone 2G11
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More Files
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Western Blot (Transfected lysate)
Western Blot analysis of PLEKHC1 expression in transfected 293T cell line by PLEKHC1 monoclonal antibody (M09), clone 2G11.
Lane 1: PLEKHC1 transfected lysate(77.9 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged FERMT2 is 1 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to FERMT2 on HeLa cell . [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant PLEKHC1.
Immunogen
PLEKHC1 (AAH17327, 1 a.a. ~ 680 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MALDGIRMPDGCYADGTWELSVHVTDLNRDVTLRVTGEVHIGGVMLKLVEKLDVKKDWSDHALWWEKKRTWLLKTHWTLDKYGIQADAKLQFTPQHKLLRLQLPNMKYVKVKVNFSDRVFKAVSDICKTFNIRHPEELSLLKKPRDPTKKKKKKLDDQSEDEALELEGPLITPGSGSIYSSPGLYSKTMTPTYDAHDGSPLSPTSAWFGDSALSEGNPGILAVSQPITSPEILAKMFKPQALLDKAKINQGWLDSSRSLMEQDVKENEALLLRFKYYSFFDLNPKYDAIRINQLYEQAKWAILLEEIECTEEEMMMFAALQYHINKLSIMTSENHLNNSDKEVDEVDAALSDLEITLEGGKTSTILGDITSIPELADYIKVFKPKKLTLKGYKQYWCTFKDTSISCYKSKEESSGTPAHQMNLRGCEVTPDVNISGQKFNIKLLIPVAEGMNEIWLRCDNEKQYAHWMAACRLASKGKTMADSSYNLEVQNILSSLKMQHLNPDPQLIPEQITTDITPECLVSPRYLKKYKNKQITARILEAHQNVAQMSLIEAKMRFIQAWQSLPEFGITHFIARFQGGKKEELIGIAYNRLIRMDASTGDAIKTWRFSNMKQWNVNWEIKMVTVEFADEVRLSFICTEVDCKVVHEFIGGYIFLSTRAKDQNESLDEEMFYKLTSGWV
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (98); Rat (98)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PLEKHC1 expression in transfected 293T cell line by PLEKHC1 monoclonal antibody (M09), clone 2G11.
Lane 1: PLEKHC1 transfected lysate(77.9 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged FERMT2 is 1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to FERMT2 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — FERMT2
Entrez GeneID
10979GeneBank Accession#
BC017327Protein Accession#
AAH17327Gene Name
FERMT2
Gene Alias
DKFZp686G11125, FLJ34213, FLJ44462, KIND2, MIG2, PLEKHC1, UNC112, UNC112B, mig-2
Gene Description
fermitin family homolog 2 (Drosophila)
Omim ID
607746Gene Ontology
HyperlinkGene Summary
family C (with FERM domain) member 1|pleckstrin homology domain containing
Other Designations
OTTHUMP00000179005|fermitin family homolog 2|kindlin 2|kindlin-2|mitogen inducible gene 2 protein|pleckstrin homology domain containing, family C (with FERM domain) member 1|pleckstrin homology domain containing, family C member 1
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Interactome
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Publication Reference
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Kindlin-2 is required for myocyte elongation and is essential for myogenesis.
Dowling JJ, Vreede AP, Kim S, Golden J, Feldman EL.
BMC Cell Biology 2008 Jul; 9:36.
Application：IF, Mouse, C2C12 cells.
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Kindlin-2 is required for myocyte elongation and is essential for myogenesis.
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