STIP1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
STIP1 transfected lysate.
Western Blot
Lane 1: STIP1 transfected lysate ( 62.6 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-STIP1 full-length
Host
Human
Theoretical MW (kDa)
62.6
Interspecies Antigen Sequence
Mouse (97); Rat (97)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-STIP1 antibody (H00010963-B01) by Western Blots.
SDS-PAGE Gel
STIP1 transfected lysate.
Western Blot
Lane 1: STIP1 transfected lysate ( 62.6 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — STIP1
Entrez GeneID
10963GeneBank Accession#
NM_006819Protein Accession#
NP_006810Gene Name
STIP1
Gene Alias
HOP, IEF-SSP-3521, P60, STI1, STI1L
Gene Description
stress-induced-phosphoprotein 1
Omim ID
605063Gene Ontology
HyperlinkGene Summary
STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).[supplied by OMIM
Other Designations
Hsp70/Hsp90-organizing protein|stress-induced-phosphoprotein 1 (Hsp70/Hsp90-organizing protein)
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Interactome
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Pathway
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Disease
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