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Last updated: 2016/12/4
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STIP1 monoclonal antibody (M35), clone 2E11

  • Catalog # : H00010963-M35
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a full-length recombinant STIP1.
  • Immunogen:
  • STIP1 (AAH02987.1, 1 a.a. ~ 543 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • MEQVNELKEKGNKALSVGNIDDALQCYSEAIKLDPHNHVLYSNRSAAYAKKGDYQKAYEDGCKTVDLKPDWGKGYSRKAAALEFLNRFEEAKRTYEEGLKHEANNPQLKEGLQNMEARLAERKFMNPFNMPNLYQKLESDPRTRTLLSDPTYRELIEQLRNKPSDLGTKLQDPRIMTTLSVLLGVDLGSMDEEEEIATPPPPPPPKKETKPEPMEEDLPENKKQALKEKELGNDAYKKKDFDTALKHYDKAKELDPTNMTYITNQAAVYFEKGDYNKCRELCEKAIEVGRENREDYRQIAKAYARIGNSYFKEEKYKDAIHFYNKSLAEHRTPDVLKKCQQAEKILKEQERLAYINPDLALEEKNKGNECFQKGDYPQAMKHYTEAIKRNPKDAKLYSNRAACYTKLLEFQLALKDCEECIQLEPTFIKGYTRKAAALEAMKDYTKAMDVYQKALDLDSSCKEAADGYQRCMMAQYNRHDSPEDVKRRAMADPEVQQIMSDPAMRLILEQMQKDPQALSEHLKNPVIAQKIQKLMDVGLIAIR
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG1 Kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00010963-M35
    Western Blot detection against Immunogen (85.25 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Flow Cytometry
  • Flow Cytometry enlargeenlarge this image
  • FACS analysis of HeLa cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained HeLa cells (Black) as negative control.
  • Flow Cytometry
  • Flow Cytometry enlargeenlarge this image
  • FACS analysis of 293 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained 293 cells (Black) as negative control.
  • Flow Cytometry
  • Flow Cytometry enlargeenlarge this image
  • FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained ES-2 cells (Black) as negative control.
  • Flow Cytometry
  • Flow Cytometry enlargeenlarge this image
  • FACS analysis of TOV-21G cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained TOV-21G cells (Black) as negative control.
  • Flow Cytometry
  • Flow Cytometry enlargeenlarge this image
  • FACS analysis of A-431 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained A-431 cells (Black) as negative control.
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • STIP1 monoclonal antibody (M35), clone 2E11. Western Blot analysis of STIP1 expression in human ovarian cancer.
  • PDF DownloadProtocol Download
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • STIP1 monoclonal antibody (M35), clone 2E11. Western Blot analysis of STIP1 expression in MCF-7.
  • PDF DownloadProtocol Download
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of STIP1 expression in transfected 293T cell line by STIP1 monoclonal antibody (M35), clone 2E11.

    Lane 1: STIP1 transfected lysate (Predicted MW: 62.6 KDa).
    Lane 2: Non-transfected lysate.
  • PDF DownloadProtocol Download
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) enlargeenlarge this image
  • Immunoperoxidase of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human ovarian cancer. [antibody concentration 0.75 ug/ml]
  • PDF DownloadProtocol Download
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Detection limit for recombinant GST tagged STIP1 is 0.1 ng/ml as a capture antibody.
  • PDF DownloadProtocol Download
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • STIP1
  • Gene Alias:
  • HOP,IEF-SSP-3521,P60,STI1,STI1L
  • Gene Description:
  • stress-induced-phosphoprotein 1
  • Gene Summary:
  • STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).[supplied by OMIM
  • Other Designations:
  • Hsp70/Hsp90-organizing protein,stress-induced-phosphoprotein 1 (Hsp70/Hsp90-organizing protein)
  • Gene Pathway
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