STIP1 monoclonal antibody (M35), clone 2E11
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant STIP1.
Immunogen
STIP1 (AAH02987.1, 1 a.a. ~ 543 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MEQVNELKEKGNKALSVGNIDDALQCYSEAIKLDPHNHVLYSNRSAAYAKKGDYQKAYEDGCKTVDLKPDWGKGYSRKAAALEFLNRFEEAKRTYEEGLKHEANNPQLKEGLQNMEARLAERKFMNPFNMPNLYQKLESDPRTRTLLSDPTYRELIEQLRNKPSDLGTKLQDPRIMTTLSVLLGVDLGSMDEEEEIATPPPPPPPKKETKPEPMEEDLPENKKQALKEKELGNDAYKKKDFDTALKHYDKAKELDPTNMTYITNQAAVYFEKGDYNKCRELCEKAIEVGRENREDYRQIAKAYARIGNSYFKEEKYKDAIHFYNKSLAEHRTPDVLKKCQQAEKILKEQERLAYINPDLALEEKNKGNECFQKGDYPQAMKHYTEAIKRNPKDAKLYSNRAACYTKLLEFQLALKDCEECIQLEPTFIKGYTRKAAALEAMKDYTKAMDVYQKALDLDSSCKEAADGYQRCMMAQYNRHDSPEDVKRRAMADPEVQQIMSDPAMRLILEQMQKDPQALSEHLKNPVIAQKIQKLMDVGLIAIR
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (97); Rat (97)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (85.25 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Flow Cytometry
FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained ES-2 cells (Black) as negative control.Flow Cytometry
FACS analysis of TOV-21G cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained TOV-21G cells (Black) as negative control.Flow Cytometry
FACS analysis of HeLa cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained HeLa cells (Black) as negative control.Flow Cytometry
FACS analysis of A-431 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained A-431 cells (Black) as negative control.Flow Cytometry
FACS analysis of 293 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained 293 cells (Black) as negative control.Western Blot (Tissue lysate)
STIP1 monoclonal antibody (M35), clone 2E11. Western Blot analysis of STIP1 expression in human ovarian cancer.Western Blot (Cell lysate)
STIP1 monoclonal antibody (M35), clone 2E11. Western Blot analysis of STIP1 expression in MCF-7.Western Blot (Transfected lysate)
Western Blot analysis of STIP1 expression in transfected 293T cell line by STIP1 monoclonal antibody (M35), clone 2E11.
Lane 1: STIP1 transfected lysate (Predicted MW: 62.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human ovarian cancer. [antibody concentration 0.75 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged STIP1 is 0.1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to STIP1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — STIP1
Entrez GeneID
10963GeneBank Accession#
BC002987Protein Accession#
AAH02987.1Gene Name
STIP1
Gene Alias
HOP, IEF-SSP-3521, P60, STI1, STI1L
Gene Description
stress-induced-phosphoprotein 1
Omim ID
605063Gene Ontology
HyperlinkGene Summary
STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).[supplied by OMIM
Other Designations
Hsp70/Hsp90-organizing protein|stress-induced-phosphoprotein 1 (Hsp70/Hsp90-organizing protein)
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Interactome
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Pathway
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Disease
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Publication Reference
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Positive associations between upregulated levels of stress-induced phosphoprotein 1 and matrix metalloproteinase-9 in endometriosis/adenomyosis.
Wang HS, Tsai CL, Chang PY, Chao A, Wu RC, Chen SH, Wang CJ, Yen CF, Lee YS, Wang TH.
PLoS One 2018 Jan; 13(1):e0190573.
Application:Cap Ab, ChIP, ELISA, IHC-P, Human, ARK2 cells, Ovarian endometriosis, Serum, Uterine adenomyosis.
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Immunohistological analysis of stress-induced phosphoprotein 1 in ovarian cancer patients with low serum cancer antigen 125 levels.
Chao A, Lee LY, Hsueh C, Lin CY, Tsai CL, Chao AS, Lin CT, Chou HH, Chang TC, Wang TH.
Taiwanese Journal of Obstetrics & Gynecology 2013 Jun; 52(2):185.
Application:IHC-P, Human, Ovarian cancer.
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Tumor Stress-Induced Phosphoprotein1 (STIP1) as a Prognostic Biomarker in Ovarian Cancer.
Chao A, Lai CH, Tsai CL, Hsueh S, Hsueh C, Lin CY, Chou HH, Lin YJ, Chen HW, Chang TC, Wang TH.
PLoS One. 2013 Feb; 8(2):e57084.
Application:IHC, Human, Human ovarian tissues.
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Stress-induced phosphoprotein 1 as a secreted biomarker for human ovarian cancer promotes cancer cell proliferation.
Wang TH, Chao A, Tsai CL, Chang CL, Chen SH, Lee YS, Chen JK, Lin YJ, Chang PY, Wang CJ, Chao AS, Chang SD, Chang TC, Lai CH, Wang HS.
Molecular & Cellular Proteomics 2010 Sep; 9(9):1873.
Application:ELISA, Human, Serum.
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Positive associations between upregulated levels of stress-induced phosphoprotein 1 and matrix metalloproteinase-9 in endometriosis/adenomyosis.
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