SEC61B 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
SEC61B transfected lysate.
Western Blot
Lane 1: SEC61B transfected lysate ( 10.00 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SEC61B full-length
Host
Human
Theoretical MW (kDa)
10
Interspecies Antigen Sequence
Mouse (98); Rat (98)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SEC61B antibody (H00010952-D01P) by Western Blots.
SDS-PAGE Gel
SEC61B transfected lysate.
Western Blot
Lane 1: SEC61B transfected lysate ( 10.00 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SEC61B
Entrez GeneID
10952GeneBank Accession#
NM_006808.2Protein Accession#
NP_006799.1Gene Name
SEC61B
Gene Alias
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Gene Description
Sec61 beta subunit
Omim ID
609214Gene Ontology
HyperlinkGene Summary
The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript. [provided by RefSeq
Other Designations
OTTHUMP00000021784|Sec61 complex, beta subunit|protein translocation complex beta|protein transport protein SEC61 beta subunit
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