PRDX3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PRDX3 transfected lysate.
Western Blot
Lane 1: PRDX3 transfected lysate ( 28.27 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PRDX3 full-length
Host
Human
Theoretical MW (kDa)
28.27
Interspecies Antigen Sequence
Mouse (85); Rat (84)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PRDX3 antibody (H00010935-B01) by Western Blots.
SDS-PAGE Gel
PRDX3 transfected lysate.
Western Blot
Lane 1: PRDX3 transfected lysate ( 28.27 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PRDX3
Entrez GeneID
10935GeneBank Accession#
NM_006793.2Protein Accession#
NP_006784.1Gene Name
PRDX3
Gene Alias
AOP-1, AOP1, MER5, MGC104387, MGC24293, PRO1748, SP-22
Gene Description
peroxiredoxin 3
Omim ID
604769Gene Ontology
HyperlinkGene Summary
This gene encodes a protein with antioxidant function and is localized in the mitochondrion. This gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000020590|antioxidant protein 1|thioredoxin-dependent peroxide reductase
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Interactome
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Disease
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