DBF4 monoclonal antibody (M01), clone 6G9
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant DBF4.
Immunogen
DBF4 (NP_006707, 2 a.a. ~ 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
NSGAMRIHSKGHFQGGIQVKNEKNRPSLKSLKTDNRPEKSKCKPLWGKVFYLDLPSVTISEKLQKDIKDLGGRVEEFLSKDISYLISNKKEAKFAQT
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (66)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.41 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of DBF4 expression in transfected 293T cell line by DBF4 monoclonal antibody (M01), clone 6G9.
Lane 1: DBF4 transfected lysate(76.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged DBF4 is approximately 0.1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to DBF4 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — DBF4
Entrez GeneID
10926GeneBank Accession#
NM_006716Protein Accession#
NP_006707Gene Name
DBF4
Gene Alias
ASK, CHIF, DBF4A, ZDBF1
Gene Description
DBF4 homolog (S. cerevisiae)
Omim ID
604281Gene Ontology
HyperlinkGene Summary
DBF-type containing 1
Other Designations
OTTHUMP00000161661|activator of S phase kinase|chiffon homolog|zinc finger, DBF-type containing 1
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Interactome
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Pathway
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Disease
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Publication Reference
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The LEDGF/p75 Integrase Binding Domain Interactome Contributes to the Survival, Clonogenicity, and Tumorsphere Formation of Docetaxel-Resistant Prostate Cancer Cells.
Greisha L Ortiz-Hernandez, Evelyn S Sanchez-Hernandez, Pedro T Ochoa, Catherine C Elix, Hossam R Alkashgari, James R W McMullen, Ubaldo Soto, Shannalee R Martinez, Carlos J Diaz Osterman, Michael Mahler, Sourav Roy, Carlos A Casiano.
Cells 2021 Oct; 10(10):2723.
Application:WB-Tr, Human, DU145, PC-3 cells.
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Transcriptional Co-activator LEDGF interacts with Cdc7-activator of S-phase kinase (ASK) and stimulates its enzymatic activity.
Hughes S, Jenkins V, Dar MJ, Engelman A, Cherepanov P.
The Journal of Biological Chemistry 2010 Jan; 285(1):541.
Application:WB-Ce, Human, HEK 293T cells.
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The LEDGF/p75 Integrase Binding Domain Interactome Contributes to the Survival, Clonogenicity, and Tumorsphere Formation of Docetaxel-Resistant Prostate Cancer Cells.
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