CPSF4 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
CPSF4 transfected lysate.
Western Blot
Lane 1: CPSF4 transfected lysate ( 27.40 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CPSF4 full-length
Host
Human
Theoretical MW (kDa)
27.4
Interspecies Antigen Sequence
Mouse (85); Rat (98)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CPSF4 antibody (H00010898-B02P) by Western Blots.
SDS-PAGE Gel
CPSF4 transfected lysate.
Western Blot
Lane 1: CPSF4 transfected lysate ( 27.40 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CPSF4
Entrez GeneID
10898GeneBank Accession#
BC050738Protein Accession#
AAH50738.1Gene Name
CPSF4
Gene Alias
CPSF30, NAR, NEB1
Gene Description
cleavage and polyadenylation specific factor 4, 30kDa
Omim ID
603052Gene Ontology
HyperlinkGene Summary
Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq
Other Designations
cleavage-polyadenylation specificity factor, 30kD|no arches-like zinc finger protein
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Interactome
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Disease
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