NPFFR2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NPFFR2 transfected lysate.
Western Blot
Lane 1: NPFFR2 transfected lysate ( 48.7 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NPFFR2 full-length
Host
Human
Theoretical MW (kDa)
48.7
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NRG4antibody (H00010886-B01P)byWesternBlots.
SDS-PAGE Gel
NPFFR2 transfected lysate.
Western Blot
Lane 1: NPFFR2 transfected lysate ( 48.7 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NPFFR2
Entrez GeneID
10886GeneBank Accession#
NM_053036Protein Accession#
NP_444264.1Gene Name
NPFFR2
Gene Alias
GPR74, NPFF2, NPGPR
Gene Description
neuropeptide FF receptor 2
Omim ID
607449Gene Ontology
HyperlinkGene Summary
This gene encodes a member of a subfamily of G-protein-coupled neuropeptide receptors. This protein is activated by the neuropeptides A-18-amide (NPAF) and F-8-amide (NPFF) and may function in pain modulation and regulation of the opioid system. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
G protein-coupled receptor 74|OTTHUMP00000160367|OTTHUMP00000160369|neuropeptide FF 2|neuropeptide G protein-coupled receptor
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Interactome
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Pathway
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Disease
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