MRPS30 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MRPS30 full-length
Host
Human
Theoretical MW (kDa)
48.4
Interspecies Antigen Sequence
Mouse (73); Rat (73)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MRPS30 antibody (H00010884-B01) by Western Blots.
Western Blot
Lane 1: MRPS30 transfected lysate ( 50.4 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
MRPS30 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MRPS30
Entrez GeneID
10884GeneBank Accession#
NM_016640Protein Accession#
NP_057724Gene Name
MRPS30
Gene Alias
DKFZp566B2024, MRP-S30, PAP, PDCD9
Gene Description
mitochondrial ribosomal protein S30
Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that is similar to the chicken pro-apoptotic protein p52. Transcript variants using alternative promoters or polyA sites have been mentioned in the literature but the complete description of these sequences is not available. [provided by RefSeq
Other Designations
(HSA)PAP protein|programmed cell death 9
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Interactome
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Disease
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