ACTL7B 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ACTL7B full-length
Host
Human
Theoretical MW (kDa)
45.2
Interspecies Antigen Sequence
Mouse (85); Rat (87)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ACTL7B antibody (H00010880-B01) by Western Blots.
SDS-PAGE Gel
ACTL7B transfected lysate.
Western Blot
Lane 1: ACTL7B transfected lysate ( 45.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ACTL7B
Entrez GeneID
10880GeneBank Accession#
NM_006686.2Protein Accession#
-Gene Name
ACTL7B
Gene Alias
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Gene Description
actin-like 7B
Omim ID
604304Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene (ACTL7B), and related gene, ACTL7A, are intronless, and are located approximately 4 kb apart in a head-to-head orientation within the familial dysautonomia candidate region on 9q31. Based on mutational analysis of the ACTL7B gene in patients with this disorder, it was concluded that it is unlikely to be involved in the pathogenesis of dysautonomia. Unlike ACTL7A, the ACTL7B gene is expressed predominantly in the testis, however, its exact function is not known. [provided by RefSeq
Other Designations
OTTHUMP00000021867|actin-like 7-beta
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