USP20 (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human USP20 full-length ORF ( NP_001008563.1, 1 a.a. - 913 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MGDSRDLCPHLDSIGEVTKEDLLLKSKGTCQSCGVTGPNLWACLQVACPYVGCGESFADHSTIHAQAKKHNLTVNLTTFRLWCYACEKEVFLEQRLAAPLLGSSSKFSEQDSPPPSHPLKAVPIAVADEGESESEDDDLKPRGLTGMKNLGNSCYMNAALQALSNCPPLTQFFLECGGLVRTDKKPALCKSYQKLVSEVWHKKRPSYVVPTSLSHGIKLVNPMFRGYAQQDTQEFLRCLMDQLHEELKEPVVATVALTEARDSDSSDTDEKREGDRSPSEDEFLSCDSSSDRGEGDGQGRGGGSSQAETELLIPDEAGRAISEKERMKDRKFSWGQQRTNSEQVDEDADVDTAMAALDQPAEAQPPSPRSSSPCRTPEPDNDAHLRSSSRPCSPVHHHEGHAKLSSSPPRASPVRMAPSYVLKKAQVLSAGSRRRKEQRYRSVISDIFDGSILSLVQCLTCDRVSTTVETFQDLSLPIPGKEDLAKLHSAIYQNVPAKPGACGDSYAAQGWLAFIVEYIRRFVVSCTPSWFWGPVVTLEDCLAAFFAADELKGDNMYSCERCKKLRNGVKYCKVLRLPEILCIHLKRFRHEVMYSFKINSHVSFPLEGLDLRPFLAKECTSQITTYDLLSVICHHGTAGSGHYIAYCQNVINGQWYEFDDQYVTEVHETVVQNAEGYVLFYRKSSEEAMRERQQVVSLAAMREPSLLRFYVSREWLNKFNTFAEPGPITNQTFLCSHGGIPPHKYHYIDDLVVILPQNVWEHLYNRFGGGPAVNHLYVCSICQVEIEALAKRRRIEIDTFIKLNKAFQAEESPGVIYCISMQWFREWEAFVKGKDNEPPGPIDNSRIAQVKGSGHVQLKQGADYGQISEETWTYLNSLYGGGPEIAIRQSVAQPLGPENLHGEQKIEAETRAV
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
128.3
Interspecies Antigen Sequence
Mouse (92); Rat (92)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — USP20
Entrez GeneID
10868GeneBank Accession#
NM_001008563.1Protein Accession#
NP_001008563.1Gene Name
USP20
Gene Alias
KIAA1003, LSFR3A, VDU2
Gene Description
ubiquitin specific peptidase 20
Gene Ontology
HyperlinkOther Designations
OTTHUMP00000022346|OTTHUMP00000022347|OTTHUMP00000022348|pVHL-interacting deubiquitinating enzyme 2|ubiquitin specific protease 20
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Interactome
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Disease
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Publication Reference
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The Deubiquitinating Enzyme USP20 Regulates ERK3 Stability and Biological Activity.
Mathien S, Déléris P, Soulez M, Voisin L, Meloche S.
Molecular and Cellular Biology 2017 Apr; 37(9).
Application:PI, WB, Human, HEK 293T cells.
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Ubiquitin-specific Peptidase 20 Regulates Rad17 Stability, Checkpoint Kinase 1 Phosphorylation and DNA Repair by Homologous Recombination.
Shanmugam I, Abbas M, Ayoub F, Mirabal S, Bsaili M, Caulder EK, Weinstock DM, Tomkinson AE, Hromas R, Shaheen M.
The Journal of Biological Chemistry 2014 Aug; 289(33):22739.
Application:Func, Human, HEK 293 cells.
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The Deubiquitinating Enzyme USP20 Regulates ERK3 Stability and Biological Activity.
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