MTHFD2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MTHFD2 transfected lysate.
Western Blot
Lane 1: MTHFD2 transfected lysate ( 26.80 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MTHFD2 full-length
Host
Human
Theoretical MW (kDa)
26.8
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MTHFD2 antibody (H00010797-D01P) by Western Blots.
SDS-PAGE Gel
MTHFD2 transfected lysate.
Western Blot
Lane 1: MTHFD2 transfected lysate ( 26.80 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MTHFD2
Entrez GeneID
10797GeneBank Accession#
NM_001040409.1Protein Accession#
NP_001035499.1Gene Name
MTHFD2
Gene Alias
NMDMC
Gene Description
methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
Omim ID
604887Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq
Other Designations
NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase|methylene tetrahydrofolate dehydrogenase (NAD+ dependent), methenyltetrahydrofolate cyclohydrolase|methylene tetrahydrofolate dehydrogenase 2
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Interactome
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Disease
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