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Last updated: 2016/12/4

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MTHFD2 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00010797-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-MTHFD2 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 27.39
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-MTHFD2 antibody (H00010797-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00010797-T01
    MTHFD2 transfected lysate.
    Western Blot
    QC Testing of H00010797-T01
    Lane 1: MTHFD2 transfected lysate ( 27.39 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • MTHFD2
  • Gene Alias:
  • NMDMC
  • Gene Description:
  • methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
  • Gene Summary:
  • This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq
  • Other Designations:
  • NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase,methylene tetrahydrofolate dehydrogenase (NAD+ dependent), methenyltetrahydrofolate cyclohydrolase,methylene tetrahydrofolate dehydrogenase 2
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