NUP50 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NUP50 transfected lysate.
Western Blot
Lane 1: NUP50 transfected lysate ( 50.1 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NUP50 full-length
Host
Human
Theoretical MW (kDa)
50.1
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NUP50 antibody (H00010762-B01) by Western Blots.
SDS-PAGE Gel
NUP50 transfected lysate.
Western Blot
Lane 1: NUP50 transfected lysate ( 50.1 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NUP50
Entrez GeneID
10762GeneBank Accession#
NM_007172Protein Accession#
NP_009103Gene Name
NUP50
Gene Alias
MGC39961, NPAP60, NPAP60L
Gene Description
nucleoporin 50kDa
Omim ID
604646Gene Ontology
HyperlinkGene Summary
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000028685|nuclear pore-associated protein 60L
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