GRAP 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
GRAP transfected lysate
Western Blot
Lane 1: GRAP transfected lysate ( 23.87 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GRAP full-length
Host
Human
Theoretical MW (kDa)
23.87
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GRAP antibody (H00010750-B01) by Western Blots.
SDS-PAGE Gel
GRAP transfected lysate
Western Blot
Lane 1: GRAP transfected lysate ( 23.87 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GRAP
Entrez GeneID
10750GeneBank Accession#
NM_006613Protein Accession#
NP_006604Gene Name
GRAP
Gene Alias
MGC64880
Gene Description
GRB2-related adaptor protein
Omim ID
604330Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the GRB2/Sem5/Drk family. This member functions as a cytoplasmic signaling protein which contains an SH2 domain flanked by two SH3 domains. The SH2 domain interacts with ligand-activated receptors for stem cell factor and erythropoietin, and facilitates the formation of a stable complex with the BCR-ABL oncoprotein. This protein also associates with the Ras guanine nucleotide exchange factor SOS1 (son of sevenless homolog 1) through its N-terminal SH3 domain. [provided by RefSeq
Other Designations
growth factor receptor-bound protein 2-related adaptor protein
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Interactome
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Disease
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