SIX2 monoclonal antibody (M01), clone 3D7
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant SIX2.
Immunogen
SIX2 (AAH24033, 1 a.a. ~ 291 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSMLPTFGFTQEQVACVCEVLQQGGNIERLGRFLWSLPACEHLHKNESVLKAKAVVAFHRGNFRELYKILESHQFSPHNHAKLQQLWLKAHYIEAEKLRGRPLGAVGKYRVRRKFPLPRSIWDGEETSYCFKEKSRSVLREWYAHNPYPSPREKRELTEATGLTTTQVSNWFKNRRQRDRAAEAKERENNENSNSNSHNPLNGSGKSVLGSSEDEKTPSGTPDHSSSSPALLLSPPPPGLPSLHSLGHPPGPSAVPVPVPGGGGADPLQHHHGLQDSILNPMSANLVDLGS
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (96); Rat (96)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (57.75 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
-
Applications
Western Blot (Transfected lysate)
Western Blot analysis of SIX2 expression in transfected 293T cell line by SIX2 monoclonal antibody (M01), clone 3D7.
Lane 1: SIX2 transfected lysate(32.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SIX2 is 0.03 ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of SIX2 over-expressed 293 cell line, cotransfected with SIX2 Validated Chimera RNAi ( Cat # H00010736-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SIX2 monoclonal antibody (M01), clone 3D7 (Cat # H00010736-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — SIX2
Entrez GeneID
10736GeneBank Accession#
BC024033Protein Accession#
AAH24033Gene Name
SIX2
Gene Alias
-
Gene Description
SIX homeobox 2
Omim ID
604994Gene Ontology
HyperlinkGene Summary
This gene is a member of the vertebrate gene family which encode proteins homologous to the Drosophila 'sine oculis' homeobox protein. The encoded protein is a transcription factor which, like other members of this gene family, may be involved in limb or eye development. [provided by RefSeq
Other Designations
sine oculis homeobox homolog 2
-
Interactome
-
Publication Reference
-
Modeled microgravity unravels the roles of mechanical forces in renal progenitor cell physiology.
Maria Elena Melica, Francesca Cialdai, Gilda La Regina, Chiara Risaliti, Tommaso Dafichi, Anna Julie Peired, Paola Romagnani, Monica Monici, Laura Lasagni.
Stem Cell Research & Therapy 2024 Jan; 15(1):20.
Application:IF, Human, Renal progenitor cells.
-
Functional differentiation and scalable production of renal proximal tubular epithelial cells from human pluripotent stem cells in a dynamic culture system.
Thao Thi Thanh Ngo, Bella Rossbach, Isabelle Sébastien, Julia C Neubauer, Andreas Kurtz, Krithika Hariharan.
Cell proliferation 2022 Mar; 55(3):e13190.
Application:Flow Cyt, Human, BCRTi005, WAe001-A, WISCi004 cells.
-
AP-2β/KCTD1 Control Distal Nephron Differentiation and Protect Against Renal Fibrosis.
Alexander G Marneros.
Developmental Cell 2020 Aug; 54(3):348.
Application:IF, IHC-Fr, Mouse, Mouse kidney.
-
The FGF, TGFβ and WNT axis Modulate Self-renewal of Human SIX2+ Urine Derived Renal Progenitor Cells.
Rahman MS, Wruck W, Spitzhorn LS, Nguyen L, Bohndorf M, Martins S, Asar F, Ncube A, Erichsen L, Graffmann N, Adjaye J.
Scientific Reports 2020 Jan; 10(1):739.
Application:Flow Cyt, Human, UF-31, UF-45, UM-27, UM-51 cells .
-
Generating Multiple Kidney Progenitors and Cell Types from Human Pluripotent Stem Cells.
Hariharan K, Reinke P, Kurtz A.
Methods in Molecular Biology (Clifton, N.J.) 2019 Feb; 1926:103.
Application:IF, Human, Renal cells.
-
A Fat4-Dchs1 signal between stromal and cap mesenchyme cells influences nephrogenesis and ureteric bud branching.
Mao Y, Francis-West P, Irvine KD.
Development 2015 Aug; 142(15):2574.
Application:IF, Mouse, Kidneys.
-
ROBO2 restricts the nephrogenic field and regulates Wolffian duct-nephrogenic cord separation.
Wainwright EN, Wilhelm D, Combes AN, Little MH, Koopman P.
Developmental Biology 2015 Aug; 404(2):88.
Application:IF, Mouse, Embryos.
-
Mdm2 is required for maintenance of the nephrogenic niche.
Hilliard SA, Yao X, El-Dahr SS.
Developmental Biology 2014 Mar; 387(1):1.
Application:IF, Mouse, Kidney.
-
SIX2 and CITED1, markers of nephronic progenitor self-renewal, remain active in primitive elements of Wilms' tumor.
Murphy AJ, Pierce J, de Caestecker C, Taylor C, Anderson JR, Perantoni AO, de Caestecker MP, Lovvorn HN 3rd.
Journal of Pediatric Surgery 2012 Jun; 47(6):1239.
Application:IHC-P, Human, Human fetal kidneys.
-
Transcription coactivator Eya2 is a critical regulator of physiological hypertrophy.
Lee SH, Kim J, Ryu JY, Lee S, Yang DK, Jeong D, Kim J, Lee SH, Kim JM, Hajjar RJ, Park WJ.
Journal of Molecular and Cellular Cardiology 2011 Dec; 52(3):718.
Application:WB-Ti, Mouse, Mouse hearts.
-
Modeled microgravity unravels the roles of mechanical forces in renal progenitor cell physiology.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com