MGEA5 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MGEA5 transfected lysate.
Western Blot
Lane 1: MGEA5 transfected lysate ( 100.87 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MGEA5 full-length
Host
Human
Theoretical MW (kDa)
100.87
Interspecies Antigen Sequence
Mouse (97); Rat (97)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MGEA5 antibody (H00010724-B01) by Western Blots.
SDS-PAGE Gel
MGEA5 transfected lysate.
Western Blot
Lane 1: MGEA5 transfected lysate ( 100.87 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MGEA5
Entrez GeneID
10724GeneBank Accession#
NM_012215.1Protein Accession#
NP_036347.1Gene Name
MGEA5
Gene Alias
FLJ11229, FLJ23355, KIAA0679, MEA5, NCOAT, OGA
Gene Description
meningioma expressed antigen 5 (hyaluronidase)
Omim ID
604039Gene Ontology
HyperlinkGene Summary
The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM
Other Designations
O-GlcNAcase|OTTHUMP00000020345|hyaluronidase in meningioma|nuclear cytoplasmic O-GlcNAcase and acetyltransferase
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Interactome
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Disease
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