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MGEA5 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00010724-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-MGEA5 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 100.87
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-MGEA5 antibody (H00010724-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00010724-T01
    MGEA5 transfected lysate.
    Western Blot
    QC Testing of H00010724-T01
    Lane 1: MGEA5 transfected lysate ( 100.87 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • MGEA5
  • Gene Alias:
  • FLJ11229,FLJ23355,KIAA0679,MEA5,NCOAT,OGA
  • Gene Description:
  • meningioma expressed antigen 5 (hyaluronidase)
  • Gene Summary:
  • The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM
  • Other Designations:
  • O-GlcNAcase,OTTHUMP00000020345,hyaluronidase in meningioma,nuclear cytoplasmic O-GlcNAcase and acetyltransferase
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