CCT6B monoclonal antibody (M01), clone 1A4
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant CCT6B.
Immunogen
CCT6B (AAH27591, 1 a.a. ~ 530 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAAIKAVNSKAEVARAQAALAVNICAARGLQDVLRTNLGPKGTMKMLASGAGDIKLTKDGNVLLDEMQIQHPTASLIAKVATAQDDVTGDGTTSNVLIIGELLKQADLYISEGLHPRIIAEGFEAAKIKALEVLEEVKVTKEMKRKILLDVARTSLQTKVHAELADVLTEVVVDSVLAVRRPGYPIDLFMVEIMEMKHKLGTDTKLIQGLVLDHGARHPDMKKRVEDAFILICNVSLEYEKTEVNSGFFYKTAEEKEKLVKAERKFIEDRVQKIIDLKDKVCAQSNKGFVVINQKGIDPFSLDSLAKHGIVALRRAKRRNMERLSLACGGMAVNSFEDLTVDCLGHAGLVYEYTLGEEKFTFIEECVNPCSVTLLVKGPNKHTLTQVKDAIRDGLRAIKNAIEDGCMVPGAGAIEVAMAEALVTYKNSIKGRARLGVQAFADALLIIPKVLAQNAGYDPQETLVKVQAEHVESKQLVGVDLNTGEPMVAADAGVWDNYCVKKQLLHSCTVIATNILLVDEIMRAGMSSLK
Host
Mouse
Reactivity
Human
Isotype
IgG2a kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (84.04 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
CCT6B monoclonal antibody (M01), clone 1A4 Western Blot analysis of CCT6B expression in HL-60 ( Cat # L014V1 ).Western Blot (Transfected lysate)
Western Blot analysis of CCT6B expression in transfected 293T cell line by CCT6B monoclonal antibody (M01), clone 1A4.
Lane 1: CCT6B transfected lysate(57.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CCT6B is approximately 0.3ng/ml as a capture antibody.ELISA
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Gene Info — CCT6B
Entrez GeneID
10693GeneBank Accession#
BC027591Protein Accession#
AAH27591Gene Name
CCT6B
Gene Alias
CCT-zeta-2, CCTZ-2, Cctz2, TCP-1-zeta-2, TSA303
Gene Description
chaperonin containing TCP1, subunit 6B (zeta 2)
Omim ID
610730Gene Ontology
HyperlinkGene Summary
This gene encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq
Other Designations
CCT-zeta-like|T-complex protein 1, zeta-2 subunit|TCP-1-zeta-like|chaperonin containing TCP1, subunit 6B|testis-specific Tcp20|testis-specific protein TSA303
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