PNMA2 monoclonal antibody (M10), clone 4F10
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant PNMA2.
Immunogen
PNMA2 (AAH62301, 1 a.a. ~ 364 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MALALLEDWCRIMSVDEQKSLMVTGIPADFEEAEIQEVLQETLKSLGRYRLLGKIFRKQENANAVLLELLEDTDVSAIPSEVQGKGGVWKVIFKTPNQDTEFLERLNLFLEKEGQTVSGMFRALGQEGVSPATVPCISPELLAHLLGQAMAHAPQPLLPMRYRKLRVFSGSAVPAPEEESFEVWLEQATEIVKEWPVTEAEKKRWLAESLRGPALDLMHIVQADNPSISVEECLEAFKQVFGSLESRRTAQVRYLKTYQEEGEKVSAYVLRLETLLRRAVEKRAIPRRIADQVRLEQVMAGATLNQMLWCRLRELKDQGPPPSFLELMKVIREEEEEEASFENESIEEPEERDGYGRWNHEGDD
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (78); Rat (80)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (65.78 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PNMA2 expression in transfected 293T cell line by PNMA2 monoclonal antibody (M10), clone 4F10.
Lane 1: PNMA2 transfected lysate (Predicted MW: 41.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of PNMA2 transfected lysate using anti-PNMA2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PNMA2 monoclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PNMA2 is approximately 3ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to PNMA2 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — PNMA2
Entrez GeneID
10687GeneBank Accession#
BC062301Protein Accession#
AAH62301Gene Name
PNMA2
Gene Alias
KIAA0883, MA2, MM2, RGAG2
Gene Description
paraneoplastic antigen MA2
Omim ID
603970Gene Ontology
HyperlinkOther Designations
onconeuronal antigen MA2|paraneoplastic neuronal antigen|retrotransposon gag domain containing 2
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Interactome
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