GNB5 monoclonal antibody (M01), clone 3A3

Catalog # H00010681-M01

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Size:100 ug
Price: USD $ 335.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Western Blot (Transfected lysate)
Application

Western Blot (Transfected lysate)

Western Blot analysis of GNB5 expression in transfected 293T cell line by GNB5 monoclonal antibody (M01), clone 3A3.

Lane 1: GNB5 transfected lysate(38.8 KDa).
Lane 2: Non-transfected lysate.

Sandwich ELISA (Recombinant protein)
Application

Sandwich ELISA (Recombinant protein)

Detection limit for recombinant GST tagged GNB5 is 0.1 ng/ml as a capture antibody.

<em>In situ</em> Proximity Ligation Assay (Cell)
Application

In situ Proximity Ligation Assay (Cell)

Proximity Ligation Analysis of protein-protein interactions between GNG7 and GNB5. HeLa cells were stained with anti-GNG7 rabbit purified polyclonal 1:1200 and anti-GNB5 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

QC Test

Western Blot detection against Immunogen (35.64 KDa) .

  • Specification

    Product Description

    Mouse monoclonal antibody raised against a partial recombinant GNB5.

    Immunogen

    GNB5 (NP_057278, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

    Sequence

    MCDQTFLVNVFGSCDKCFKQRALRPVFKKSQQLSYCSTCAEIMATEGLHENETLASLKSEAESLKGKLEEERAKLHDVELHQVAERVEAL

    Host

    Mouse

    Reactivity

    Human

    Interspecies Antigen Sequence

    Mouse (99)

    Isotype

    IgG1 Kappa

    Quality Control Testing

    Antibody Reactive Against Recombinant Protein.

    Western Blot detection against Immunogen (35.64 KDa) .

    Storage Buffer

    In 1x PBS, pH 7.4

    Storage Instruction

    Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot (Transfected lysate)

    Western Blot analysis of GNB5 expression in transfected 293T cell line by GNB5 monoclonal antibody (M01), clone 3A3.

    Lane 1: GNB5 transfected lysate(38.8 KDa).
    Lane 2: Non-transfected lysate.

    Western Blot (Recombinant protein)

    Sandwich ELISA (Recombinant protein)

    Detection limit for recombinant GST tagged GNB5 is 0.1 ng/ml as a capture antibody.

    ELISA

    In situ Proximity Ligation Assay (Cell)

    Proximity Ligation Analysis of protein-protein interactions between GNG7 and GNB5. HeLa cells were stained with anti-GNG7 rabbit purified polyclonal 1:1200 and anti-GNB5 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
  • Gene Info — GNB5

    Entrez GeneID

    10681

    GeneBank Accession#

    NM_016194

    Protein Accession#

    NP_057278

    Gene Name

    GNB5

    Gene Alias

    FLJ37457, FLJ43714, GB5

    Gene Description

    guanine nucleotide binding protein (G protein), beta 5

    Omim ID

    604447

    Gene Ontology

    Hyperlink

    Gene Summary

    Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternatively spliced transcript variants encoding different isoforms exist. [provided by RefSeq

    Other Designations

    G protein, beta subunit 5L|G protein, beta-5 subunit|guanine nucleotide-binding protein, beta subunit 5L|guanine nucleotide-binding protein, beta-5 subunit|transducin beta chain 5

  • Interactome
  • Pathway
  • Disease
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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