RGS14 293T Cell Transient Overexpression Lysate(Denatured)

Catalog # H00010636-T01

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Size:100 uL
Price: USD $ 247.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
SDS-PAGE Gel
QC Test

SDS-PAGE Gel

RGS14 transfected lysate.

Western Blot
QC Test

Western Blot

Lane 1: RGS14 transfected lysate ( 61.4 KDa)
Lane 2: Non-transfected lysate.

  • Specification

    Transfected Cell Line

    293T

    Plasmid

    pCMV-RGS14 full-length

    Host

    Human

    Theoretical MW (kDa)

    61.4

    Quality Control Testing

    Transient overexpression cell lysate was tested with Anti-RGS14 antibody (H00010636-B01P) by Western Blots.

    SDS-PAGE Gel

    RGS14 transfected lysate.

    Western Blot

    Lane 1: RGS14 transfected lysate ( 61.4 KDa)
    Lane 2: Non-transfected lysate.

    Storage Buffer

    1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

    Storage Instruction

    Store at -80°C. Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot

  • Gene Info — RGS14

    Entrez GeneID

    10636

    GeneBank Accession#

    NM_006480

    Protein Accession#

    NP_006471.2

    Gene Name

    RGS14

    Gene Alias

    -

    Gene Description

    regulator of G-protein signaling 14

    Omim ID

    602513

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene encodes a member of the regulator of G-protein signaling family. This protein contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq

    Other Designations

    regulator of G-protein signalling 14

  • Interactome
  • Disease
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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