TAF6L 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TAF6L full-length
Host
Human
Theoretical MW (kDa)
50.6
Interspecies Antigen Sequence
Mouse (93); Rat (93)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TAF6L antibody (H00010629-B01) by Western Blots.
SDS-PAGE Gel
TAF6L transfected lysate.
Western Blot
Lane 1: TAF6L transfected lysate ( 50.6 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TAF6L
Entrez GeneID
10629GeneBank Accession#
BC008785.2Protein Accession#
AAH08785.1Gene Name
TAF6L
Gene Alias
FLJ11136, MGC4288, PAF65A
Gene Description
TAF6-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kDa
Omim ID
602946Gene Ontology
HyperlinkGene Summary
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a protein that is a component of the PCAF histone acetylase complex and structurally similar to one of the histone-like TAFs, TAF6. The PCAF histone acetylase complex, which is composed of more than 20 polypeptides some of which are TAFs, is required for myogenic transcription and differentiation. [provided by RefSeq
Other Designations
TAF6-like RNA polymerase II|TAF6-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65 kD|p300/CBP-associated factor (PCAF)-associated factor 65
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Interactome
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Pathway
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