POLR3F 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
POLR3F transfected lysate.
Western Blot
Lane 1: POLR3F transfected lysate ( 34.87 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-POLR3F full-length
Host
Human
Theoretical MW (kDa)
34.87
Interspecies Antigen Sequence
Mouse (98); Rat (99)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-POLR3F antibody (H00010621-B01) by Western Blots.
SDS-PAGE Gel
POLR3F transfected lysate.
Western Blot
Lane 1: POLR3F transfected lysate ( 34.87 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — POLR3F
Entrez GeneID
10621GeneBank Accession#
NM_006466.2Protein Accession#
NP_006457.2Gene Name
POLR3F
Gene Alias
MGC13517, RPC39, RPC6
Gene Description
polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. [provided by RefSeq
Other Designations
DNA-directed RNA polymerase III 39 kDa polypeptide F|DNA-directed RNA polymerases III 39 kDa polypeptide|OTTHUMP00000030359|RNA polymerase III C39 subunit
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Interactome
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Pathway
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Disease
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