POLR3F (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human POLR3F full-length ORF ( NP_006457.2, 1 a.a. - 316 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MAEVKVKVQPPDADPVEIENRIIELCHQFPHGITDQVIQNEMPHIEAQQRAVAINRLLSMGQLDLLRSNTGLLYRIKDSQNAGKMKGSDNQEKLVYQIIEDAGNKGIWSRDIRYKSNLPLTEINKILKNLESKKLIKAVKSVAASKKKVYMLYNLQPDRSVTGGAWYSDQDFESEFVEVLNQQCFKFLQSKAETARESKQNPMIQRNSSFASSHEVWKYICELGISKVELSMEDIETILNTLIYDGKVEMTIIAAKEGTVGSVDGHMKLYRAVNPIIPPTGLVRAPCGLCPVFDDCHEGGEISPSNCIYMTEWLEF
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
62.1
Interspecies Antigen Sequence
Mouse (98); Rat (99)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — POLR3F
Entrez GeneID
10621GeneBank Accession#
NM_006466.2Protein Accession#
NP_006457.2Gene Name
POLR3F
Gene Alias
MGC13517, RPC39, RPC6
Gene Description
polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. [provided by RefSeq
Other Designations
DNA-directed RNA polymerase III 39 kDa polypeptide F|DNA-directed RNA polymerases III 39 kDa polypeptide|OTTHUMP00000030359|RNA polymerase III C39 subunit
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Interactome
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Pathway
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Disease
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