AHSA1 monoclonal antibody (M01), clone 1A2-A8
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant AHSA1.
Immunogen
AHSA1 (AAH00321, 1 a.a. ~ 338 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAKWGEGDPRWIVEERANATNVNNWHWTERDASNWSTDKLKTLFLAVQVQNEEGKCEVTEVSKLDGEASINNRKGKLIFFYEWSVKLNWTGTSKSGVQYKGHVEIPNLSGENSVDEVEISVSLAKDEPDTNLVALMKEEGVKLLREAMGIYISTLKTEFTQGMILPTMNGESVDPVGQPALKTEERKAKPAPSKTQARPVGVKIPTCKITLKETFLTSPEELYRVFTTQELVQAFTHAPATLEADRGGKFHMVDGNVSGEFTDLVPEKHIVMKWRFKSWPEGHFATITLTFIDKNGETELCMEGRGIPAPEEERTRQGWQRYYFEGIKQTFGYGARLF
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (94); Rat (94)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (62.92 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
AHSA1 monoclonal antibody (M01), clone 1A2-A8 Western Blot analysis of AHSA1 expression in HepG2 ( Cat # L019V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged AHSA1 is 3 ng/ml as a capture antibody.ELISA
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Gene Info — AHSA1
Entrez GeneID
10598GeneBank Accession#
BC000321Protein Accession#
AAH00321Gene Name
AHSA1
Gene Alias
AHA1, C14orf3, p38
Gene Description
AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast)
Omim ID
608466Gene Ontology
HyperlinkGene Summary
O
Other Designations
AHA1, activator of heat shock 90kDa protein ATPase homolog 1
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Interactome
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Publication Reference
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The CFTR-Associated Ligand Arrests the Trafficking of the Mutant ΔF508 CFTR Channel in the ER Contributing to Cystic Fibrosis.
Bergbower E, Boinot C, Sabirzhanova I, Guggino W, Cebotaru L.
Cellular Physiology and Biochemistry 2018 Jan; 45(2):639.
Application:WB, Human, CFBE41o- cells.
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delta9-THC increases endogenous AHA1 expression in rat cerebellum and may modulate CB1 receptor function during chronic use.
Filipeanu CM, Guidry JJ, Leonard ST, Winsauer PJ.
Journal of Neurochemistry 2011 Sep; 118(6):1101.
Application:WB-Ti, WB-Tr, Human, Rat, HEK 293T cells, Rat cerebellum.
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High-content functional screen to identify proteins that correct F508del-CFTR function.
Trzcinska-Daneluti A, Ly D, Huynh L, Jiang C, Fladd C, Rotin D.
Molecular & Cellular Proteomics 2008 Dec; 8(4):780.
Application:WB, Human, 293MSR-GT cells.
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Characterizing the role of Hsp90 in production of heat shock proteins in motor neurons reveals a suppressive effect of wild-type Hsf1.
Taylor DM, Tradewell ML, Minotti S, Durham HD.
Cell Stress & Chaperones 2007 Jun; 12(2):151.
Application:IF, Mouse, Dorsal root ganglia.
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The CFTR-Associated Ligand Arrests the Trafficking of the Mutant ΔF508 CFTR Channel in the ER Contributing to Cystic Fibrosis.
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