MRPL28 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MRPL28 full-length
Host
Human
Theoretical MW (kDa)
28.27
Interspecies Antigen Sequence
Mouse (85); Rat (84)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MRPL28 antibody (H00010573-B02) by Western Blots.
SDS-PAGE Gel
MRPL28 transfected lysate.
Western Blot
Lane 1: MRPL28 transfected lysate ( 28.27 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MRPL28
Entrez GeneID
10573GeneBank Accession#
NM_006428.3Protein Accession#
NP_006419.2Gene Name
MRPL28
Gene Alias
MAAT1, MGC8499, p15
Gene Description
mitochondrial ribosomal protein L28
Omim ID
604853Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion. [provided by RefSeq
Other Designations
OTTHUMP00000067337|melanoma antigen p15|melanoma-associated antigen recognised by cytotoxic T lymphocytes
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