ARFGEF1 monoclonal antibody (M02), clone 3E11
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant ARFGEF1.
Immunogen
ARFGEF1 (NP_006412.2, 311 a.a. ~ 411 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
EVLYDGENHDCEEKPQDIVQNIVEEMVNIVVGDMGEGTTINASADGNIGTIEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (84); Rat (84)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.85 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ARFGEF1 is 0.1 ng/ml as a capture antibody.ELISA
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Gene Info — ARFGEF1
Entrez GeneID
10565GeneBank Accession#
NM_006421Protein Accession#
NP_006412.2Gene Name
ARFGEF1
Gene Alias
ARFGEP1, BIG1, D730028O18Rik, DKFZP434L057, P200
Gene Description
ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited)
Omim ID
604141Gene Ontology
HyperlinkGene Summary
ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP. It contains a Sec7 domain, which may be responsible for the guanine-nucleotide exchange activity and also the brefeldin A inhibition. [provided by RefSeq
Other Designations
brefeldin A-inhibited guanine nucleotide-exchange protein 1
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Interactome
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Disease
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