ANP32B monoclonal antibody (M05), clone 8E10
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of ANP32B expression in transfected 293T cell line by ANP32B monoclonal antibody (M05), clone 8E10.
Lane 1: ANP32B transfected lysate (Predicted MW: 28.8 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ANP32B is 0.3 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to ANP32B on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (55.2 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant ANP32B.
Immunogen
ANP32B (NP_006392.1, 1 a.a. ~ 251 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MDMKRRIHLELRNRTPAAVRELVLDNCKSNDGKIEGLTAEFVNLEFLSLINVGLISVSNLPKLPKLKKLELSENRIFGGLDMLAEKLPNLTHLNLSGNKLKDISTLEPLKKLECLKSLDLFNCEVTNLNDYRESVFKLLPQLTYLDGYDREDQEAPDSDAEVDGVDEEEEDEEGEDEEDEDDEDGEEEEFDEEDDEDEDVEGDEDDDEVSEEEEEFGLDEEDEDEDEDEEEEEGGKGEKRKRETDDEGEDD
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (55.2 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of ANP32B expression in transfected 293T cell line by ANP32B monoclonal antibody (M05), clone 8E10.
Lane 1: ANP32B transfected lysate (Predicted MW: 28.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ANP32B is 0.3 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to ANP32B on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — ANP32B
Entrez GeneID
10541GeneBank Accession#
NM_006401.1Protein Accession#
NP_006392.1Gene Name
ANP32B
Gene Alias
APRIL, PHAPI2, SSP29
Gene Description
acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
Gene Ontology
HyperlinkGene Summary
O
Other Designations
OTTHUMP00000021759|OTTHUMP00000063770|acidic protein rich in leucines
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