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DCTN2 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00010540-T02
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-DCTN2 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 44.2
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-DCTN2 antibody (H00010540-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00010540-T02
    DCTN2 transfected lysate.
    Western Blot
    QC Testing of H00010540-T02
    Lane 1: DCTN2 transfected lysate ( 44.20 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • DCTN2
  • Gene Alias:
  • DCTN50,DYNAMITIN,RBP50
  • Gene Description:
  • dynactin 2 (p50)
  • Gene Summary:
  • This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. [provided by RefSeq
  • Other Designations:
  • 50 kD dynein-associated polypeptide,dynactin 2,dynactin complex 50 kD subunit
  • Gene Pathway
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