DCTN2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
DCTN2 transfected lysate.
Western Blot
Lane 1: DCTN2 transfected lysate ( 44.20 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DCTN2 full-length
Host
Human
Theoretical MW (kDa)
44.2
Interspecies Antigen Sequence
Mouse (94); Rat (95)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DCTN2 antibody (H00010540-D01P) by Western Blots.
SDS-PAGE Gel
DCTN2 transfected lysate.
Western Blot
Lane 1: DCTN2 transfected lysate ( 44.20 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DCTN2
Entrez GeneID
10540GeneBank Accession#
BC000718Protein Accession#
AAH00718.1Gene Name
DCTN2
Gene Alias
DCTN50, DYNAMITIN, RBP50
Gene Description
dynactin 2 (p50)
Omim ID
607376Gene Ontology
HyperlinkGene Summary
This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. [provided by RefSeq
Other Designations
50 kD dynein-associated polypeptide|dynactin 2|dynactin complex 50 kD subunit
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