RNASEH2A monoclonal antibody (M01), clone 3G5-F5
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant RNASEH2A.
Immunogen
RNASEH2A (AAH11748, 1 a.a. ~ 299 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MDLSELERDNTGRCRLSSPVPAVCRKEPCVLGVDEAGRGPVLGPMVYAICYCPLPRLADLEALKVADSKTLLESERERLFAKMEDTDFVGWALDVLSPNLISTSMLGRVKYNLNSLSHDTATGLIQYALDQGVNVTQVFVDTVGMPETYQARLQQSFPGIEVTVKAKADALYPVVSAASICAKVARDQAVKKWQFVEKLQDLDTDYGSGYPNDPKTKAWLKEHVEPVFGFPQFVRFSWRTAQTILEKEAEDVIWEDSASENQEGLRKITSYFLNEGSQARPRSSHRYFLERGLESATSL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Rat (84)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (58.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
RNASEH2A monoclonal antibody (M01), clone 3G5-F5 Western Blot analysis of RNASEH2A expression in Jurkat ( Cat # L017V1 ).Western Blot (Transfected lysate)
Western Blot analysis of RNASEH2A expression in transfected 293T cell line by RNASEH2A monoclonal antibody (M01), clone 3G5-F5.
Lane 1: RNASEH2A transfected lysate(33.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RNASEH2A is 1 ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of RNASEH2A over-expressed 293 cell line, cotransfected with RNASEH2A Validated Chimera RNAi ( Cat # H00010535-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RNASEH2A monoclonal antibody (M01), clone 3G5-F5 (Cat # H00010535-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — RNASEH2A
Entrez GeneID
10535GeneBank Accession#
BC011748Protein Accession#
AAH11748Gene Name
RNASEH2A
Gene Alias
AGS4, JUNB, RNASEHI, RNHIA, RNHL
Gene Description
ribonuclease H2, subunit A
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a component of the heterotrimeric type II ribonuclease H enzyme (RNAseH2). RNAseH2 is the major source of ribonuclease H activity in mammalian cells and endonucleolytically cleaves ribonucleotides. It is predicted to remove Okazaki fragment RNA primers during lagging strand DNA synthesis and to excise single ribonucleotides from DNA-DNA duplexes. Mutations in this gene cause Aicardi-Goutieres Syndrome (AGS), a an autosomal recessive neurological disorder characterized by progressive microcephaly and psychomotor retardation, intracranial calcifications, elevated levels of interferon-alpha and white blood cells in the cerebrospinal fluid
Other Designations
ribonuclease H2, large subunit|ribonuclease HI, large subunit
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Interactome
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Pathway
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Publication Reference
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Antiribonuclease H2 antibodies are an immune biomarker for systemic lupus erythematosus.
Nozawa K, Doe K, Uomori K, Sekigawa I, Takasaki Y, Yamaji K, Tamura N.
Autoimmunity 2017 May; 50(4):241.
Application:WB, Human, Serum from patients with systemic lupus erythematosus.
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Antiribonuclease H2 antibodies are an immune biomarker for systemic lupus erythematosus.
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