CHERP monoclonal antibody (M01), clone 2H5
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant CHERP.
Immunogen
CHERP (AAH21294.1, 795 a.a. ~ 883 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
GSNSAPPIPDSRLGEENKGHQMLVKMGWSGSGGLGAKEQGIQDPIKGGDVRDKWDQYKGVGVALDDPYENYRRNKSYSFIARMKARDEC
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (94); Rat (94)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.53 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
CHERP monoclonal antibody (M01), clone 2H5 Western Blot analysis of CHERP expression in IMR-32 ( Cat # L008V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CHERP is 0.3 ng/ml as a capture antibody.ELISA
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Gene Info — CHERP
Entrez GeneID
10523GeneBank Accession#
NM_006387Protein Accession#
AAH21294.1Gene Name
CHERP
Gene Alias
DAN16, SCAF6, SRA1
Gene Description
calcium homeostasis endoplasmic reticulum protein
Gene Ontology
HyperlinkOther Designations
ERPROT213-21|SR-related CTD associated factor 6|protein with polyglutamine repeat
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Interactome
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Publication Reference
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Nuclear ALG-2 protein interacts with Ca2+ homeostasis endoplasmic reticulum protein (CHERP) Ca2+-dependently and participates in regulation of alternative splicing of inositol trisphosphate receptortype 1 (IP3R1) pre-mRNA.
Sasaki-Osugi K, Imoto C, Takahara T, Shibata H, Maki M.
The Journal of Biological Chemistry 2013 Nov; 288(46):33361.
Application:IF, IP-WB, WB-Ce, Human, HEK 293T, HeLa cells.
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Nuclear ALG-2 protein interacts with Ca2+ homeostasis endoplasmic reticulum protein (CHERP) Ca2+-dependently and participates in regulation of alternative splicing of inositol trisphosphate receptortype 1 (IP3R1) pre-mRNA.
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