UBE2E3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
UBE2E3 transfected lysate
Western Blot
Lane 1: UBE2E3 transfected lysate ( 22.77 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-UBE2E3 full-length
Host
Human
Theoretical MW (kDa)
22.77
Interspecies Antigen Sequence
Mouse (100); Rat (100)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-UBE2E3 antibody (H00010477-B01) by Western Blots.
SDS-PAGE Gel
UBE2E3 transfected lysate
Western Blot
Lane 1: UBE2E3 transfected lysate ( 22.77 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — UBE2E3
Entrez GeneID
10477GeneBank Accession#
NM_006357Protein Accession#
NP_006348Gene Name
UBE2E3
Gene Alias
UBCH9, UbcM2
Gene Description
ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast)
Omim ID
604151Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
ubiquitin carrier protein|ubiquitin-conjugating enzyme E2E 3|ubiquitin-conjugating enzyme E2E 3 (homologous to yeast UBC4/5)|ubiquitin-protein ligase
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Interactome
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Pathway
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Disease
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