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UBE2E3 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00010477-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-UBE2E3 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 22.77
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-UBE2E3 antibody (H00010477-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00010477-T01
    UBE2E3 transfected lysate
    Western Blot
    QC Testing of H00010477-T01
    Lane 1: UBE2E3 transfected lysate ( 22.77 KDa).
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • UBE2E3
  • Gene Alias:
  • UBCH9,UbcM2
  • Gene Description:
  • ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast)
  • Gene Summary:
  • The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
  • Other Designations:
  • ubiquitin carrier protein,ubiquitin-conjugating enzyme E2E 3,ubiquitin-conjugating enzyme E2E 3 (homologous to yeast UBC4/5),ubiquitin-protein ligase
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