ATP5H 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ATP5H transfected lysate.
Western Blot
Lane 1: ATP5H transfected lysate ( 18.5 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ATP5H full-length
Host
Human
Theoretical MW (kDa)
18.5
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ATP5H antibody (H00010476-B01P) by Western Blots.
SDS-PAGE Gel
ATP5H transfected lysate.
Western Blot
Lane 1: ATP5H transfected lysate ( 18.5 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ATP5H
Entrez GeneID
10476GeneBank Accession#
NM_006356Protein Accession#
NP_006347Gene Name
ATP5H
Gene Alias
ATP5JD, ATPQ
Gene Description
ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d
Gene Ontology
HyperlinkGene Summary
Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, F0, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The F0 seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the F0 complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15. [provided by RefSeq
Other Designations
ATP synthase D chain, mitochondrial|ATP synthase, H+ transporting, mitochondrial F1F0, subunit d|My032 protein
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