TADA3L 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
TADA3L transfected lysate.
Western Blot
Lane 1: TADA3L transfected lysate ( 41.4 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TADA3L full-length
Host
Human
Theoretical MW (kDa)
41.4
Interspecies Antigen Sequence
Mouse (99); Rat (98)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TADA3L antibody (H00010474-D01P) by Western Blots.
SDS-PAGE Gel
TADA3L transfected lysate.
Western Blot
Lane 1: TADA3L transfected lysate ( 41.4 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TADA3L
Entrez GeneID
10474GeneBank Accession#
NM_133480.1Protein Accession#
NP_597814.1Gene Name
TADA3L
Gene Alias
ADA3, FLJ20221, FLJ21329, hADA3
Gene Description
transcriptional adaptor 3 (NGG1 homolog, yeast)-like
Omim ID
602945Gene Ontology
HyperlinkGene Summary
Many DNA-binding transcriptional activator proteins enhance the initiation rate of RNA polymerase II-mediated gene transcription by interacting functionally with the general transcription machinery bound at the basal promoter. Adaptor proteins are usually required for this activation, possibly to acetylate and destabilize nucleosomes, thereby relieving chromatin constraints at the promoter. The protein encoded by this gene is a transcriptional activator adaptor and has been found to be part of the PCAF histone acetylase complex. In addition, it associates with the tumor suppressor protein p53 and is required for full activity of p53 and p53-mediated apoptosis. At least four alternatively spliced variants have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq
Other Designations
transcriptional adaptor 3-like
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Interactome
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Disease
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