LYPLA1 monoclonal antibody (M05), clone 3D5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of LYPLA1 expression in transfected 293T cell line by LYPLA1 monoclonal antibody (M05), clone 3D5.
Lane 1: LYPLA1 transfected lysate(24.7 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged LYPLA1 is 0.1 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to LYPLA1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (35.2 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant LYPLA1.
Immunogen
LYPLA1 (AAH08652.1, 66 a.a. ~ 151 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
NVAMPSWFDIIGLSPDSQEDESGIKQAAENIKALIDQEVKNGIPSNRIILGGFSQGGALSLYTALTTQQKLAGVTALSCWLPLRAS
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (91); Rat (92)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.2 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of LYPLA1 expression in transfected 293T cell line by LYPLA1 monoclonal antibody (M05), clone 3D5.
Lane 1: LYPLA1 transfected lysate(24.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged LYPLA1 is 0.1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to LYPLA1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — LYPLA1
Entrez GeneID
10434GeneBank Accession#
BC008652Protein Accession#
AAH08652.1Gene Name
LYPLA1
Gene Alias
APT-1, LPL1, LYSOPLA
Gene Description
lysophospholipase I
Omim ID
605599Gene Ontology
HyperlinkGene Summary
Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene hydrolyzes lysophosphatidylcholine in both monomeric and micellar forms. The use of alternate polyadenylation sites has been found for this gene. There are alternatively spliced transcript variants described for this gene but the full length nature is not known yet. [provided by RefSeq
Other Designations
acyl-protein thioesterase-1|lysophospholipase 1|lysophospholipid-specific lysophospholipase
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Interactome
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Pathway
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Publication Reference
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Thioesterase activity and subcellular localization of acylprotein thioesterase 1/lysophospholipase 1.
Hirano T, Kishi M, Sugimoto H, Taguchi R, Obinata H, Ohshima N, Tatei K, Izumi T.
Biochimica et Biophysica Acta 2009 Aug; 1791(8):797.
Application：WB-Re, Recombinant protein.
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Thioesterase activity and subcellular localization of acylprotein thioesterase 1/lysophospholipase 1.
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