KNTC2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NDC80 transfected lysate.
Western Blot
Lane 1: NDC80 transfected lysate ( 70.73 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-KNTC2 full-length
Host
Human
Theoretical MW (kDa)
70.73
Interspecies Antigen Sequence
Mouse (83); Rat (84)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-KNTC2 antibody (H00010403-B01) by Western Blots.
SDS-PAGE Gel
NDC80 transfected lysate.
Western Blot
Lane 1: NDC80 transfected lysate ( 70.73 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NDC80
Entrez GeneID
10403GeneBank Accession#
NM_006101Protein Accession#
NP_006092Gene Name
NDC80
Gene Alias
HEC, HEC1, KNTC2, TID3, hsNDC80
Gene Description
NDC80 homolog, kinetochore complex component (S. cerevisiae)
Omim ID
607272Gene Ontology
HyperlinkGene Summary
HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM
Other Designations
highly expressed in cancer, rich in leucine heptad repeats|kinetochore associated 2
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Interactome
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Disease
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